The reproductive process in teleost fish is controlled by the hypothalamic-pituitary-gonadal axis, being activated by external factors such as water temperature and photoperiod. However, most fish in captivity do not spawn due to blockages at different levels of the gonadal maturation process, particularly in rheophilic species such as pacu (Piaractus mesopotamicus). In this sense, different procedures are used in order to stimulate the reproductive...