1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments Douglas W. Houston 2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos 3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos Michael D. Sheets