Therapeutic Proteins: Methods and Protocols

Book Overview

1. Therapeutic Proteins

Dimiter S. Dimitrov

2. Synthetic Antibody Libraries

Bryce Nelson and Sachdev S. Sidhu

3. The Construction of 'Phylomer' Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions

Nadia Milech and Paul Watt

4. Ribosome Display and Screening for Protein Therapeutics

Damjana Kastelic and Mingyue He

5. Yeast Display of Engineered Antibody Domains

Qi Zhao, Zhongyu Zhu, and Dimiter S. Dimitrov

6. Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains

Rui Gong, Weizao Chen, and Dimiter S. Dimitrov

7. Engineering of Affibody Molecules for Therapy and Diagnostics

Joachim Feldwisch and Vladimir Tolmachev

8. Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination

Karl J.M. Hanf

9. Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting

Enrico DiGiammarino, Tariq Ghayur, and Junjian Liu

10. Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris

Steve Schoonooghe

11. Use of E. coli for the Production of a Single Protein

Lili Mao and Masayori Inouye

12. Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli

Brent L. Nannenga and Fran?ois Baneyx

13. Transient Expression Technologies: Past, Present, and Future

Sabine Geisse and Bernd Voedisch

14. Stable Transfection Pools for Large Quantity of Protein Production

Jianxin Ye

15. Mammalian Stable Expression of Biotherapeutics

Thomas Jostock and Hans-Peter Knopf

16. Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed

Cory L. Nykiforuk and Joseph G. Boothe

17. Methods for Chromatographic Removal of Endotoxin

Adam J. Lowe, Cameron L. Bardliving, and Carl A. Batt

18. Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps

Dana Cipriano, Michael Burnham, and Joseph V. Hughes

19. High-Throughput Quantitative N-Glycan Analysis of Glycoproteins

Margaret Doherty, Ciara A. McManus, Rebecca Duke, and Pauline M. Rudd

20. High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown, Khanita Karaveg, Stephen Hamilton, and Huijuan Li

21. Databases and Tools in Glycobiology

Natalia V. Artemenko, Andrew G. McDonald, Gavin P. Davey, and Pauline M. Rudd

22. Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS

Lihua Huang and P. Clayton Gough

23. Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry

Jennifer Zhang and Viswanatham Katta

24. Monitoring of Subvisible Particles in Therapeutic Proteins

Satish K. Singh and Maria R. Toler

25. Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms

Erinc Sahin and Christopher J. Roberts

26. Computational Methods to Predict Therapeutic Protein Aggregation

Patrick M. Buck, Sandeep Kumar, Xiaoling Wang, Neeraj J. Agrawal, Bernhardt L. Trout, and Satish K. Singh

27. Coarse-Grained Simulations of Protein Aggregation

Troy Cellmer and Nicolas L.

• Format: Paperback
• Language: English
• ISBN: 1493959492
• ISBN13: 9781493959495
• Release Date: August 2016
• Publisher: Humana
• Length: 502 Pages
• Weight: 1.95 lbs.
• Dimensions: 1.0" x 7.0" x 10.0"