Protein Microarray for Disease Analysis: Methods and Protocols

By Xiaohong Wen

Part I: Protein-Detecting Analytical Microarrays

1. Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays

Tanya Knickerbocker and Gavin MacBeath

2. Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening

Chen Li and David M. Lubman

3. Antibody Suspension Bead Arrays

Jochen M. Schwenk and Peter Nilsson

4. Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies

V?ctor J. Cid, Ekkehard Kauffmann, and Mar?a Molina

5. Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays

Nicholas O. Fischer and Theodore M. Tarasow

6. Recombinant Lectin Microarrays for Glycomic Analysis

Daniel C. Propheter, Ku-Lung Hsu, and Lara K. Mahal

Part II: Antigen Microarrays for Immunoprofiling

7. Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection

Persis P. Wadia, Bita Sahaf, and David B. Miklos

8. SPOT Synthesis as a Tool to Study Protein-Protein Interactions

Dirk F.H. Winkler, Heiko Andresen, and Kai Hilpert

9. Native Antigen Fractionation Protein Microarrays for Biomarker Discovery

Robert J. Caiazzo, Jr., Dennis J. O'Rourke, Timothy J. Barder, Bryce P. Nelson, and Brian C.-S. Liu

10. Immunoprofiling Using NAPPA Protein Microarrays

Sahar Sibani and Joshua LaBaer

Part III: Protein Function Microarrays

11. High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA)

Andrea Fiebitz and Dominique Vanhecke

12. Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System

Kalavathy Sitaraman and Deb K. Chatterjee

13. Kinase Substrate Interactions

Michael G. Smith, Jason Ptacek, and Michael Snyder

14. A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues

Jun Seop Jeong, Hee-Sool Rho, and Heng Zhu

Part IV: Strategies for Validation of Candidate Targets

15. Multiplexed Detection of Antibodies Using Programmable Bead Arrays

Karen S. Anderson

16. A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays

Ovidiu Marina, Jonathan S. Duke-Cohan, and Catherine J. Wu

Part V: Generation of Proteomic Libraries

17. Development of Expression-Ready Constructs for Generation of Proteomic Libraries

Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, and Susan E. Celniker

Part VI: Detection Methods

18. Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection

Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, and Virginia Espina

19. F?rster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays

Michael Sch?ferling and Stefan Nagl

20. Label-Free Detection with Surface Plasmon Resonance Imaging

Christopher Lausted, Zhiyuan Hu, and

• Format: Paperback
• Language: English
• ISBN: 1493961748
• ISBN13: 9781493961740
• Release Date: August 2016
• Publisher: Humana
• Length: 373 Pages
• Weight: 1.48 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"