Recombinant and in Vitro RNA Synthesis: Methods and Protocols

Book Overview

1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold

Luc Ponchon and Fr?d?ric Dardel

2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold

Luc Ponchon, Genevi?ve Beauvais, Sylvie Nonin-Lecomte and Fr?d?ric Dardel

3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR

Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams

4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE

Jo L. Linpinsel and Graeme L. Conn

5. Preparation of Short RNA by in vitro Transcription

Cheng Lu and Pingwei Li

6. Native RNA Purification by Gel Filtration Chromatography

Evan P. Booy, Hui Meng and Sean A. McKenna

7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends

Johanna M. Avis, Graeme L. Conn, and Scott C. Walker

8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends

Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka

9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods

Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong

10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA

Genevi?ve Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault

11. Affinity Purification of RNA using an ARiBo Tag

Genevi?ve Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompr?-Brodeur, Vanessa Delfosse and Pascale Legault

12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments

Virginia K. Vachon and Graeme L. Conn

13. In Vitro Transcription of Modified RNAs

Stephanie L. Moon and Jeffrey Wilusz

14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis

N. Ruth Zearfoss and Sean P. Ryder

15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA

Ya-Ming Hou

16. Se-derivatized RNAs for X-ray Crystallography

Lina Lin and Zhen Huang

17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs

Luigi Martino and Maria R Conte

18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC

Pierre Dagenais and Pascale Legault

19. Splint Ligation of RNA with T4 DNA Ligase

Christopher J. Kershaw and Raymond T. O'Keefe

• Format: Paperback
• Language: English
• ISBN: 1493958305
• ISBN13: 9781493958306
• Release Date: August 2016
• Publisher: Humana
• Length: 274 Pages
• Weight: 1.12 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"