Novel Polymeric Biochips for Enhanced Detection of Infectious Diseases

by Fatimah Ibrahim and Samira Hosseini

1 Current optical biosensors in clinical practice
1.1 Biosensors
1.2 Enzyme-linked immunosorbent assay (ELISA)
1.3 Sandwich ELISA
1.3.1 Capture antibody immobilization
1.3.2 Washing procedure
1.3.3 Blocking step
1.3.4 Virus attachment
1.3.5 Primary antibody attachment
1.3.6 Secondary labeled antibody attachment and recording the signals
1.3.7 Evaluation of ELISA
1.4 Advantages and disadvantages of ELISA
1.5 Surface functionalization
References
2 Alternative chemical approach
2.1 Proposed chemical alternative
2.2 Characterization techniques
2.2.1 Morphology analysis by scanning electron microscopy (SEM)
2.2.2 Topography analysis by atomic force microscopy (AFM)
2.2.3 Water-in-air contact angle measurement
2.3 Dengue fever
References
3 Biochips Fabrication and surface characterization
3.1 Fabrication of the biochips
3.2 Morphology analysis of the biochips
3.3 Topography analysis of the biochips
3.4 Water-in-air contact angle measurement on the surface of biochips
References
4 Application of the biochips in dengue virus detection
4.1 Sandwich ELISA
4.2 Dengue virus detection in sandwich ELISA assay
4.3 Physical immobilization of the target analyte on the biochips and performance of the assay in dengue virus detection
4.3.1 Detection range study
4.3.2 Calibration of the assay
4.3.3 Evaluation of the assay
4.4 Covalent immobilization of the target analyte on the biochips and performance of the assay in dengue virus detection
4.5 Immobilization of the target analyte by using amine-bearing spacers
References
Summary
Appendix

• Format: Paperback
• Language: English
• ISBN: 9811001065
• ISBN13: 9789811001062
• Release Date: November 2015
• Publisher: Springer
• Length: 55 Pages
• Weight: 0.24 lbs.
• Dimensions: 0.1" x 6.1" x 9.2"