Molecular characterization of CA125 in Benign and Malignant Ovarian Tumors: CA125 in Tumors

by Prof Sami A. AL-Mudhaffar Dr.

Immunoradiomatric assay technique was used to measure the level of serum tumor markers CA125 and CEA in 30 healthy women, 24 patients with benign ovarian tumors and 34 patients with malignant ovarian tumors. Upon comparison of the results, the CA125 level was found above normal (35u.ml-1) in 80% of patients with ovarian cancer, while CEA level was elevated in 44% of patients with ovarian cancer (more than 3.0 u.ml-1). Among the pre-menopausal women, the values of CA125 and CEA in sera of patients with malignant ovarian tumors were found to be significantly higher (P<0.05) than their corresponding values in sera of healthy and that in patients with benign tumors. The mean value of CA125 tumor marker in relation to menopausal status of ovarian cancer women, was found to be significantly higher (P<0.05) in post-menopausal patients, than that in pre-menopausal patients, while there were no differences found in the CEA value between the two statuses.Modified immunoradiometric assay (IRMA) was used to characterize the binding of 125I-antiCA125 antibody to CA125 in the supernatant fraction of ovarian tumor homogenates. Different factors affecting this binding were extensively studied such as pH, time, temperature, concentration of salts, concentration of antibodies and concentration of antigensCA125 of homogenate obtained from post-menopausal patients with malignant ovarian tumor was partially purified by gel filtration technique. Two forms of CA125 were found (BI and BII) with molecular weights 670 and 100 kDa respectively. The binding characteristics of the partial purified CA125 with 125I-antiCA125 antibody were investigated.Kinetic parameters of the binding of 125I-antiCA125 antibody with CA125 of homogenate obtained from pre-menopausal patients with benign and malignant tumors, post-menopausal patients with malignant tumors, and partially purified CA125, at different temperatures were determined. The results indicated that the binding reaction was time and temperature dependent process and it follows second order rate law in all studied groups.The thermodynamics of the binding of 125I-antiCA125 antibody with CA125 of homogenate obtained from pre-menopausal patients with benign and malignant tumors, post-menopausal patients with malignant tumors, and the partially purified CA125, at different temperatures were determined. All studied groups showed exothermic and spontaneous reactions and the participation of enthalpy was low. Thermodynamic parameters of transition state (DH*, DG*, DS*) and activation energy (Ea) were also determined. Spectroscopic studies, in the U.V region, were carried out on the complex (125I-antiCA125 antibody/CA125) formed of the partially purified CA125, from post-menopausal patients with malignant ovarian tumors, to its specific antibody. Different factors affecting the absorption band such as pH, polarity and denaturation agents were extensively studied. The heat stability and spectrophotometric pH titration of the complex were also included in this work.

• Format: Paperback
• Language: English
• ISBN: 1523373253
• ISBN13: 9781523373253
• Release Date: January 2016
• Publisher: Createspace Independent Publishing Platform
• Length: 178 Pages
• Weight: 0.94 lbs.
• Dimensions: 0.4" x 8.5" x 11.0"