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Hardcover Methods of Protein Microcharacterization: A Practical Handbook Book

ISBN: 0896030903

ISBN13: 9780896030909

Methods of Protein Microcharacterization: A Practical Handbook

Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti- tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach...

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