Inositol Phosphates and Lipids: Methods and Protocols

By Christopher J. Barker

Book Overview

Table of Contents Preface Contributors 1. The Role of Inositol and the Principals of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells Christopher J. Barker and Per-Olof Berggren 2. HPLC Separation of Inositol Polyphosphates Christopher J. Barker, Christopher Illies, and Per-Olof Berggren 3. Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells James C. Otto and John D. York 4. A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine 5. Synthesis of InsP7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) Cristina Azevedo, Adam Burton, Matthew Bennett, Sara Maria Nancy Onnebo, and Adolfo Saiardi 6. Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP7) J. Kent Werner, Jr., Traci Speed, and Rashna Bhandari 7. Synthesis and Non-Radioactive Micro-Analysis of Diphosphoinositol Phosphates by HPLC with Post-Column Complexometry Hongying Lin, Karsten Lindner, and Georg W. Mayr 8. Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio Andreas Nagel, Christopher J. Barker, Per-Olof Berggren, and Christopher Illies 9. Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference Akihiro Mizutani, Kastuhiro Kawaai, Chihiro Hisatsune, Hideaki Ando, Takayuki Michikawa, and Katsuhiko Mikoshiba 10. Phosphoinositide-Specific Phospholipase C b1 Signal Transduction in the Nucleus Roberta Fiume, Gabriella Teti, Irene Faenza, and Lucio Cocco 11. Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides Nullin Divecha 12. Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC Frank T. Cooke 13. Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry Trevor R. Pettitt 14. Imaging Phosphoinositide Dynamics in Living Cells Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm

• Format: Paperback
• Language: English
• ISBN: 1617796891
• ISBN13: 9781617796890
• Release Date: July 2012
• Publisher: Humana
• Length: 230 Pages
• Weight: 0.98 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"