Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols

Book Overview

1. Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research

Catherine M. With, David L. Evers, and Jeffrey T. Mason

2. Tissue Microarrays: Construction and Uses

Carol B. Fowler, Yan-Gao Man, Shimin Zhang, Timothy J. O'Leary, Jeffrey T. Mason, and Robert E. Cunningham

3. Standardization in Immunohistology

Anthony S-Y Leong and Trishe Y-M Leong

4. Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue

David Robertson and Clare M. Isacke

5. Microwaves for Chromogenic-in-situ-hybridization

Anthony S-Y Leong and Zenobia Haffajee

6. Automated Analysis of FISH-stained HER2/neu Samples with Metafer

Christian Schunck and Eiman Mohammad

7. Laser Capture Microdissection (LCM) of FFPE Tissue Sections

Bridging the Gap between Microscopy and Molecular Analysis

Renate Burgemeister

8. Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections

Renate Burgemeister

9. Microarray-based CGH and Copy Number Analysis of FFPE Samples

Fahd Al-Mulla

10. Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform

Marianne Tuefferd, An de Bondt, Ilse Van den Wyngaert, Willem Talloen, and Hinrich G?hlmann

11. Whole Genome Amplification of DNA Extracted From FFPE Tissues

Mira Bosso and Fahd Al-Mulla

12. Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue

Brendan Doyle, Ciar?n O'Riain, and Kim Appleton

13. Analysis of DNA Methylation in FFPE Tissues using the MethyLight Technology

Ashraf Dallol, Waleed Al- Ali, Amina Al-Shaibani, and Fahd Al-Mulla

14. RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions

Mei-Lan Liu, Jennie Jeong, Ranjana Ambannavar, Carl Millward, Frederick Baehner, Chithra Sangli, Debjani Dutta, Mylan Pho, Anhthu Nguyen, and Maureen T. Cronin

15. RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues

Aaron Scott, Ranjana Ambannavar, Jennie Jeong, Mei-Lan Liu and Maureen T. Cronin

16. microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues

Aihua Liu and Xiaowei Xu

17. Gene Expression Profiling of RNA Extracted from FFPE tissues: NuGEN Technologies' Whole-Transcriptome Amplification System

Leah Turner, Joe Don Heath, and Nurith Kurn

18. Protein Mass Spectrometry Applications on FFPE Tissue Sections

Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason

19. An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2?-CS100 Approach

Am?lie Denou?l, Florence Boissi?re-Michot, Philippe Rochaix, Fr?d?ric Bibeau, Nathalie Boulle

• Format: Paperback
• Language: English
• ISBN: 1493961713
• ISBN13: 9781493961719
• Release Date: August 2016
• Publisher: Humana
• Length: 312 Pages
• Weight: 1.25 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"