Base Editors: Methods and Protocols

Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes

1. Introduction and Perspectives of DNA Base Editors

Beomjong Song and Sangsu Bae

2. Web-Based Computational Tools for Base Editors

Gue-Ho Hwang and Sangsu Bae

3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE

Jinman Park and Hui Kwon Kim

4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq

Daesik Kim

Part II: Application of BEs for Disease Modeling

5. Base Editing of Human Hematopoietic Stem Cells

Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer

6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells

Xiaohui Zhang, Yuting Guan, and Dali Li

7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors

Ji-Eun See and Yongsub Kim

8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice

Hiroki Sasaguri

9. Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim

Part III: Therapeutic Base Editing Strategies

10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor

Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo

11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors

Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj

12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

Dong Yang and Chun-Qing Song

13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors

Yohan Kim, Jaemin Jeong, and Dongho Choi

14. Application of Base Editor-Mediated Genome Editing in Mouse Retina

Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim

Part IV: Use of BEs in Plants and Bacteria

15. High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice

Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou

16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate

Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim

17. Cytosine Base Editing in Bacteria

• Format: Hardcover
• Language: English
• ISBN: 107162878X
• ISBN13: 9781071628782
• Release Date: January 2023
• Publisher: Humana
• Length: 235 Pages
• Weight: 1.43 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"