The objective of the study was to compare the frequency of contamination on used with unused (brand new) healing abutment after sterilization using Phloxine B dye and to determine the contamination on different sites of healing abutment using Phloxine B dye. A total of 60 healing abutment (Zimmer and Biohorizon) divided into equal test (unused new HA) and control groups (used HA) were evaluated for contamination after sterilization. Data was analyzed using SPSS version 22. Chi-square was used to compare the frequency of contamination between two groups. Effect modifiers like site of healing abutment and type of healing abutment were controlled through stratification. The level of significance was kept at p value = 0.01).
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