Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro toplasts of rice, potato, soybean,...
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