This book constitutes the thoroughly refereed post-proceedings of the 7th International Bi-Conference Workshop on Agent-Oriented Information Systems, AOIS 2005, held in Utrecht, Netherlands, in July 2005 and in Klagenfurt, Austria, in October 2005. The 19 revised full papers are organized in topical sections on agent behavior, communications and reasoning, methodologies and ontologies, agent-oriented software engineering, as well as applications.